![]() ![]() Led a project on epithelial morphogenesis using D organoid cultures of MDCK cysts, resulting in submitted manuscript collaborated with teammates on a project resulting in publication and $k grant funding.Organized and flexible, perform and oversee multiple individual tasks simultaneously, ensuring quality and efficiency while remaining within deadlinesįluorescence-activated cell sorting (FACS)īioluminescence resonance energy transfer (BRET)įast protein liquid chromatography (FPLC)Įpithelial morphogenesis - Pr.The J3-SNAP fusion protein was subsequently purified by IMAC. coli BL21-DE3 was confirmed by SDS-PAGE and Western blot analysis. Results Successful expression of J3-SNAP in E. In a confocal microscope, the illumination and detection optics are focused on the same diffraction-limited spot in. Cell-surface binding of SNAP-Surface® Alexa Fluor® 488 -conjugated J3-SNAP on Env expressing HEK293Tcells was assessed by confocal microscopy analysis. This technique allows for high-resolution imaging in thick tissues. Completed projects through teamwork and collaborations, being regarded as a positive and cooperative spirit Confocal microscopy provides a means of rejecting the out-of-focus light from the detector such that it does not contribute blur to the images being collected.Independent design, execution, analysis, and reporting of research results, resulting in $k funding, published reviews, award for best conference presentation.Technical skills and research experience applicable to target identification and mechanism-of-action/pathway analysis, compound screening, development of complex cellular models and cellular assays.Epithelial cell biology scientist with a knack for solving complex problems and a strong record of research accomplishments resulting in peer-reviewed publications.After logging in, come back to this page and refresh your browser. Inoculated seedling root sections were prepared as described above. Small (3 l) volumes of cells were mounted on microscope slides. Only with the advent of VTRs in the 1980s and computers capable of processing images, in the early 1990s there was a real opportunity to create and effectively apply those modern microscopes that are used in our time.This button will open the login/register page in a new tab. Fluorescence Microscopy and Confocal Microscopy To image bacterial cells, cultures were grown overnight in LB containing 80 g/mL tetracycline, washed once in nutrient solution and resuspended in the same medium. By removing or approximating the structure to the object, the depth of the optical section of the tissue being examined can be varied. When the disc rotates quickly, the fragments are added to the overall picture. The invention was based on the ability of light, passing through the smallest holes in the disk and the magnifying lens, to penetrate into the depth of the tissue and illuminate the cell fragment at a distance from the surface. ![]() This disc was invented in 1883 by a German student Paul Nipkov, in honor of which he received his name - the Nipkov disk (or nipkov disk). To measure the fluorescence lifetime of H2B-eGFP in HeLaH2B-2FP nuclei, we used two-photon excitation on a laser-scanning confocal microscope equipped with. The main element of modern microscopes was designed at the end of the XIX century and was a rotating disk with the smallest holes spirally located. The idea of creating a microscope capable at the cellular level to show an intravital cut of living tissue was actively developed 130 years ago. ![]()
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